Bacteria Growth in Pipelines

Bacterial growth in process pipelines happens when operating and material conditions allow microorganisms to attach, survive, and multiply (often as biofilms). The main causes can be grouped as follows:




1️⃣ Water & Nutrient Availability (Primary Cause)

Bacteria need water + food to grow.

Presence of water or moisture (especially stagnant or low-flow zones)

Organic matter (oils, hydrocarbons, sugars, proteins)

Inorganic nutrients (iron, sulfur, nitrogen, phosphorus)

Dissolved oxygen (for aerobic bacteria) or sulfates (for SRB)


📌 Even “clean” water can support bacteria if nutrients are present at low levels.




2️⃣ Flow Conditions

Poor hydraulic conditions strongly promote growth.

Low flow velocity

Dead legs, blind branches, unused connections

Stagnant zones (shutdowns, batch operation)

Laminar flow instead of turbulent flow


➡ These allow bacteria to settle and attach to pipe walls.




3️⃣ Pipe Material & Surface Condition

Some materials promote attachment more than others.

Carbon steel & cast iron → rough surface, iron acts as nutrient

Corroded or pitted pipes

Weld seams and gaskets

Rubber liners and elastomers


🦠 Rough surfaces make it easier for bacteria to form biofilms.




4️⃣ Temperature Range

Most process bacteria thrive in:

20–45 °C → ideal for many bacteria

<60 °C → biofilm still possible

>70 °C → many bacteria die (not all)


⚠ Thermophilic bacteria can survive at higher temperatures in some systems.




5️⃣ Chemical Conditions

Certain chemistry favors bacterial growth:

Low disinfectant residual (chlorine, biocide, ozone)

Neutral pH (6–8) → optimal

Low salinity (some bacteria tolerate high salinity)

Sulfates → support sulfate-reducing bacteria (SRB)





6️⃣ Lack of Proper Disinfection / Biocide Control

No continuous or periodic biocide dosing

Incorrect biocide type or concentration

Poor mixing or contact time

Biocide neutralized by organic load


➡ Bacteria adapt and form resistant biofilms.




7️⃣ Oxygen Conditions

Different bacteria dominate depending on oxygen presence:

Aerobic bacteria → oxygen present

Anaerobic bacteria → oxygen-free zones (dead legs, deposits)

Sulfate-reducing bacteria (SRB) → major cause of MIC corrosion





8️⃣ Contamination Sources

Bacteria can be introduced from:

Makeup water

Poorly cleaned new pipes

Maintenance activities

Open tanks and vents

Recycled or reused process water





9️⃣ Deposits & Fouling

Sediments, scale, corrosion products

Sludge and oil films


These shield bacteria from disinfectants and create micro-environments.




⚠ Consequences of Bacterial Growth

Microbiologically Influenced Corrosion (MIC)

Flow restriction and pressure drop

Product contamination

Heat transfer loss

Bad odor (H₂S)

Reduced pipe life





✅ Typical Prevention Measures

Eliminate dead legs

Maintain minimum flow velocity

Proper material selection (e.g., stainless steel, plastics)

Regular pigging / flushing

Correct biocide selection & dosing

Maintain disinfectant residual

Control nutrients and deposits

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